ASTM F / FM – Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using. ASTM F / FM – ASTM International (ASTM). Title. “Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by . STANDARD USED: ASTM F, Standard Test Method for Resistance of Materials Used in Protective. Clothing to Penetration by Blood-Borne Pathogens .

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Do not use Google Chrome Contact. The method described in these standards, evaluates the effectiveness of materials used in the manufacture of protective garments against bloodborne pathogens using a surrogate virus suspended in a liquid, simulating the spontaneous contact conditions in a sanitary environment.

Some studies, however, suggest that mechanical pressures exceeding kPa [50 psig] can occur during actual clinical use 56. This test method is based on Test Method F for measuring resistance of chemical protective clothing materials to penetration by liquids. In one of the 60 mL compartments capacity the viral suspension of exposure is introduced, containing the bacteriophage Phi-X and in the opposite compartment the appearance of liquid or the presence of the virus is detected.

To help simulate the wetting characteristics of blood and body fluids, the surface tension of the Phi-X Bacteriophage challenge suspension is adjusted to approximate the lower end of this surface tension range. The test includes a positive control consisting of a membrane with a pore slightly greater than the diameter of the bacteriophage 0.

Clothing for protection against contact with blood and body fluids — Determination of resistance of protective clothing materials to penetration by blood-borne pathogens – Test method using Phi-X bacteriophage.

If you not change browser settings, you agree to it. The visual detection technique of this test method is supplemented with a biologically based assay capable of detecting virus under the specified test conditions. Active view current version of standard.

ASTM International – Standard References for ASTM F / FM – 13

The suspension used to prepare the suspension of bacteriophage use contains 3. Referenced Documents purchase separately The documents listed below are referenced within the subject standard but f6171 not provided as part of the standard. A precision and bias statement shall be reported at the end of a test method.

Prewetting agents, such as alcohol, and contaminating agents, such as perspiration, may also compromise the integrity of the protective barrier. The resulting surface tension of the Phi-X Bacteriophage challenge suspension is approximately 0. The type must be specified. To perform the method, samples of the materials are exposed to a nutrient broth containing the bacteriophage virus Phi-X, as a virus surrogated by its characteristics similar in size to the hepatitis C virus, and by extension to the hepatitis B virus and of human immunodeficiency virus these viruses are not used because of their difficulty to be cultured in laboratories.


Because of the length of time required to complete this method, it may not be suitable for use as a material or protective clothing quality control or quality assurance procedure. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. The test is performed in a chamber of two compartments, separated by the material evaluated. These diseases can be caused by a wide variety of microorganisms and can pose a significant risk to health and life.

To protect themselves from infectious agents asrm by blood, health personnel should wear protective clothing made of materials that prevent microbes or viruses from passing through. In case a non-sterile material is used, the test will include the corresponding controls to exclude the presence of the bacteriophage in the materials evaluated.

This is accomplished by adding surfactant to the Phi-X Bacteriophage nutrient broth.

This standard does not purport to address all of the safety concerns, if any, associated with asgm use. Skip directly to search Skip directly to A to Z list Skip directly to navigation Skip directly to page options Skip directly to site content.

Health professionals who treat and care for patients can be exposed to biological fluids that can transmit diseases. The method is evaluated by two procedures: Historical Version s – view previous versions of standard. Please see the full standard for complete details. Materials passing Test Method F should then be tested against bacteriophage penetration using this test method to verify performance. Inferences for protection from other pathogens must be assessed on a case-by-case basis.

The pieces of the evaluated materials are recommended to have about 75 mm on each side, of which a 57 mm diameter circle is exposed. The values stated in each system must be used independently of the other, without combining values in any way. A retaining screen is used to support the specimen. The values stated in each system must be used independently of the other, without combining values in any way.

The test is carried out observing the penetration of liquid without pressure for 5 minutes, followed by 1 minute at the indicated pressure 1. The material used in the protective garment will pass or fail in the test, depending on whether it resists penetration is not traversed or allows it to be traversed.

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Glove directives and norms list

This test method does not address the design, overall construction r1671 components, or interfaces of garments or other factors which may affect the overall protection offered by the protective clothing. If these conditions are of concern, the performance of protective clothing materials should be evaluated for Phi-X Bacteriophage penetration following an appropriate preconditioning technique representative of the expected conditions of use.


Many factors can effect the wetting and penetration characteristics of body fluids, such as: These protective garments can be, for example, gloves, aprons, masks, hoods, or boots, among others. The possible alterations of the protective material due to physical, chemical or thermal effects should be taken into account, since in this case they would negatively influence the behavior of the material, so that in case it can occur, the tests should be carried out before exposures to such material, physical, chemical or thermal conditions that may deteriorate it.

The manufacturer must indicate the possibility that the material under test can be altered by thermal treatment wet sterilizationso as not to do so in the event that it can be altered. Examples of body protection include laboratory coats, coveralls, vests, jackets, aprons, surgical gowns and full body suits Medical protective clothing.

This includes bullet-proof clothing, general protective clothing and full body ensembles that protect from cuts, radiation, temperature extremes, hot splashes from molten metals and other hot liquids, potential impacts from tools, machinery and materials and hazardous chemicals.

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A definitive procedure that produces a test result: Trace Laboratories – Denver, CO, The surface tension range for blood and body fluids excluding saliva is approximately 0. Any visual or virological evidence of penetration will indicate the inability of the material to resist penetration.

Glove directives and norms list – SHIELD Scientific : SHIELD Scientific

This test method is normally used to evaluate specimens from individual finished items of protective clothing and individual samples of materials that aatm candidates for items of protective clothing.

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The exposure is carried out for a specified time and under a selected pressure.