ANTIMICROBIAL ACTIVITY OF LEUCAS ASPERA PDF

Both the extracts exhibited a significant antibacterial activity against all the screened microorganisms. Key words: Leucas aspera, Antibacterial activity. Leucas aspera, Leucas zeylanica, antifungal, dermatophytes. RESULTS: Antifungal activity of Leucas sp. against Dermatophytes. Objective: The aim of this study was to explicate antibacterial, antifungal, and antioxidant activities of Leucas aspera flowers. Methods: Antibacterial activity was.

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A review of the literature. Antifungal activity was also done by agar diffusion method.

Antioxidant and antibacterial activity of different parts of Leucas aspera

The percentage of scavenging of all the plant extracts root, flower, leaf and stem was compared with positive controls. Phytochemical screening Phytochemical screening of the both plant extracts were carried out as per the methods and tests given by Collins and Lyne to decipher the presence or absence of various phyto compounds. The major chemical substances of interest in these surveys were the alkaloids and steroidal sapogenins, however other diverse groups of naturally occurring phytocomponents such as flavanoids, tannins, unsaturated sterols.

Herbarium has been deposited in the departmental herbarium. In recent years microorganisms have developed resistance to many commercial antibiotics due to indiscriminate use of antimicrobial drugs and this has created immense clinical problem in the treatment of infectious diseases 1.

The activity against both types of bacteria may be indicative of the presence of broad spectrum of antibiotic compounds or simply general metabolic toxin [20]. Microorganisms Gram positive Staphylococcus aureus S. How to cite this article: One approach might be the testing of plants traditionally used for their antifungal activities as potential sources for drug development 3.

Clinically isolated strains of dermatophytes culture were obtained from Department of Microbiology, Presidency College, Chennai and were used for the antifungal assay. Figure 2 was obtained by plotting a graph of percentage scavenging against the log concentration of the root samples. F and Riley T.

Sources of industrial and medicinal materials. It should be emphasized that toxic effect of the antioxidant and antimicrobial agent on the host cells must be considered, as a substance may exhibit antioxidant and antimicrobial activity by virtue of its toxic effect on the cells [22].

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Antimicrobial effects of finnish plant extracts containing flavonoids and other phenolic compounds. Nevertheless, data obtained leucaas the results are, in general agreement with other authors 15, 16, Vitaceae and Cyperus rotundus L.

Swati Paul, Dibyajyoti Saha. Since the LC 50 values for both the 12 h and 24 h observation were higher than 1. This was done for all four types of plant extracts as well as methanol negative control.

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IC 50 value of the root extract Based on the screening results, it was indicated that the root had the highest scavenging activity which was The present finding suggests that the methanol root extract of L.

These results imply that the ethanolic root extract shows slightly higher radical scavenging activity which may be attributed to its stronger proton-donating abilities. Biological effects of essential oils- review. Apart from that, research done by Mangathayaru et al [21] indicated that the methanol extract of L. On the other hand Trichophyton mentagrophytes, Candida albicans, Aspergillus flavus and Penicillium sp. Advances in cancer therapy with plant based natural products.

Many reports of antibacterial activity of plants extracts against human pathogens and their pharmaceutical application are available.

Antimicrobial activity of lecuas Indian medicinal plants used in folkloric medicine.

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J Plant Taxono ; Highest activity was recorded against penicillin by the methanol extract of Leucas zeylanica with zone of inhibition of 10mm which is nearer to control where the control clotrimazele showed an inhibition zone of The leaves are also used as insecticides and mosquito repellent in rural area 6.

The methanol extracts were subjected to antioxidant, antimicrobial and brine shrimp lethality assay.

The methanol extract of Leucas aspera evaluated for antifungal activity is found to have higher range of zone of inhibition for Trichophyton mentagrophytes, Epidermophyton floccosum, Candida tropicalis, Microsporum if, Microsporum nanum, Aspergillus flavus and Penicillium sp. Similarly the study performed by 13 showed the antifungal activity of ethanol and methanol extract of Spilanthes paniculata against Aspergillusniger, at the leucaz time it is sspera stated in the above study that ethyl acetate extract of Leucas aspera also showed the antifungal effect against Aspergillusniger, where as in this experiment methanol extract of leaf of Leucas aspera and Leucas zeylanica exhibited antfungal activity against Aspergillus flavus which is reported for the first time.

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These bacteria were chosen to be studied as they are important pathogens and also due to rapidly developed antibiotic resistance as antibiotic use increases. The IC 50 value 6. From the study it is evident that Leucas aspera and Leucas zeylanica possess potential antidermatophytic activity and further study on these plants may lead to explore novel bioactive compounds.

In case of chloroform the highest activity was also seen against the growth of P. The results observed at 12 h and 24 h indicated that the brine shrimp sensitivity to root extract was dose dependent.

Brine shrimp lethality bioassay of root extract Brine shrimp lethality bioassay was carried out according to Meyer et al [16] to investigate the cytotoxicity of the root extract. London, Butter Worths and Co. At a higher concentration, these extracts may exhibit more significant free radical scavenging activity.

Gram positive Staphylococcus aureus S. The results indicate that the root extract possess the highest antioxidant as well as antimicrobial activity. The percentage yield of filtrate extract was calculated and subjected to preliminary phytochemicals analysis. University of Karachi Department of Botany; Asiatic Soc of Bangladesh; Antifungal activity of three Chilean plant extracts on Botrytis cinerea.

Removal of dry plant parts was done by filtration through cheesecloth and Whatman filter paper. Plant extracts showing antimicrobial activity against certain pathogens have been reported by various leuvas 25, 26, 27, 28, 29, In – vitro fungi: Antifungal activity of the clove essential oil from Syzygiumaromaticum on Candida, Aspergillus antimicdobial dermatophytes species. High reduction of DPPH is related to the high azpera activity performed by particular sample. Chemical constituents and uses.